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Elaine Tam
Co-op Student
Contact information
Department of Biochemistry and Molecular Biology
Michigan State University
East Lansing, MI 48824-1319
elainet_@hotmail.com or tam@msu.edu
Education
BSc. Honours Biochemistry (1999 – present)
University of Waterloo, Canada
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Research
As a co-op student in Tim Zacharewski’s lab I have been
working under two of his graduate students, Cora Fong and Darrell
Boverhof.
With Cora, I am preparing plasmid samples for microarray printing
of human arrays. This means I get to practice my pipetting skills,
since this involves the PCR amplification of the plasmids, checking
the amplification by gel electrophoresis, filtering and re-suspending
the PCR product, and condensing the 96-well plates into 384-well
plates for printing. There is also a final check on every tenth
re-suspended plate. In total there are 111 plates for the human
set. During the summer months, a rat set will also be prepared.
I am also working under the supervision of Darrell for various
projects all of which deal with Cyp1a1 genes and how
TCDD treatment will affect their expression. While working with
Darrell, I have been able to have hands on experience with reverse
transcriptions, real-time PCR, ligation reactions/transformations,
growing and treating MCF-7 cells, and harvesting RNA from tissue
and cells. During my summer months here, I may also be able
to do some work with mice.
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