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Research Project
My current research project involves the study of changes
in gene expression in the uteri of immature, ovariectomized
Sprague Dawley rats exposed to ethynyl estradiol (EE), a synthetic,
orally active estrogen. A time course study was performed, in
which rats were dosed by oral gavage with 100 ug/kg EE b.w.
once daily for 3 consecutive days and were sacrificed at 2,
4, 8, 12, 18, 24, 72, 84, 96, 120, 144, and 168 hours following
the initial dose. I am using customized rat cDNA spotted microarrays
with ~8,500 features to examine changes in gene expression in
order to find temporally active genes involved in mediating
the uterotropic response, as well as the uterine regression
following peak induction.
Once we understand the gene expression profiles associated with
normal estrogen signaling we can better understand the mechanisms
of endocrine disrupting chemicals and molecules which mediate
their toxicity through the estrogen receptor(s).
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