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Research Projects
The project I’m currently working on is the "Comparative
Analysis of AhR-Mediated Gene Expression Responses Elicited
by TCDD and Indolo(3,2-b)carbazole in Murine Hepatoma Cells
". This project involved conducting a dose-response and
time course experiment with ICZ in Hepa1c1c7 cells. For the
dose-response, cells were dosed with 0.01, 0.1, 1, 10, 100 and
1000 nM ICZ and harvested after 12 hours. For the time course,
cells were dosed with 1000 nM ICZ and harvested 1, 2, 4, 8,
12, 24 and 48 hours after treatment. The global gene expression
changes elicited by ICZ were quantified using custom cDNA microarrays
containing 13,361 features. The results of these studies are
being characterized and compared against the responses elicited
by TCDD in order to identify compound-specific and compound
conserved responses mediated.
Other projects that I have been involved with include:
• Verified gene expressions responses in Hepa1c1c7 elicited
by BNF through quantitative real-time PCR
• Participated in the harvesting of tissues for in vivo
studies
• Assisted in the oligonucleotide expansion of the rat
cDNA microarray platform
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