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Research Project
As an undergraduate lab assistant in the Zacharewski Lab, I
work primarily with graduate student Joshua
Kwekel. I am currently working on clone amplification for
spotted-cDNA microarrays generated in the lab. Most of my work
consists of high-throughput PCR amplification of both rat and
mouse clones, and gel electrophoresis confirmation of clone
identity. I am also learning about microarrays as a tool for
gene expression profiling as well as real-time PCR confirmation
of interesting genes. I’ve assisted in RNA isolation and
quantification from whole tissues in preparation for microarray
and real-time experiments.
I have some experience in cell culture work and I am planning
to work on a project to identify the functionality of estrogen
receptor alpha (ERa) in the H4IIE (rat hepatoma) cell line (a
potential in vitro model for the lab). I will use real-time
PCR analysis of E2 responsive genes to determine if H4IIE’s
contain a functional ERa.
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