Rebecca Rotman
Co-op Student

Contact information
Department of Biochemistry and Molecular Biology
Michigan State University
East Lansing, MI 48824-1319
rotmanr@msu.edu

Education
B.Math, Hon. Computers Science (Bioinformatics Option) (1999-Present)
University of Waterloo

 

Work at Zacharewski Laboratory

During my first co-op term in the Zacharewski lab, my work involved both the development of in silico application and wet lab experiments under the guidance of Lyle Burgoon.

In Silico Projects The Real-Time PCR Interface is an application which allows the user to input and query primers, cDNA samples (from RNA reverse transcription), Real-Time PCR plate information, Real-Time PCR SDS files, and Real-Time PCR expression data. This application is divided into three components: Primers, BioSample cDNA, and Real-Time PCR Experiments. Data for each of these components can be inserted and queried using the application via the user interface (for a single record/query), an input file, or in a batch submission (a directory of input files).

The Real-Time PCR Experiments component allows the users to separately input the Real-Time PCR plate information, Real-Time PCR SDS files, and Real-Time PCR expression data. The separate insertion of the data allows members of the lab to insert Real-Time PCR plate information, experiment results, and analyze of the data at separate times. The query functionality of the Real-Time PCR Experiment component gives the user the ability to search for the results for a specific plate, gene, bioSample cDNA, SDS file, and much more.

Wet Lab Projects

My wet lab projects involve sequence verifying clones on our human microarray to assess gene identity and function, as well as creating 96 well plates for Real-Time PCR experiments.