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Interest and work experience
Work at Bio products laboratory involved purification, characterization,
formulation and stability of monoclonal and recombinant anti-bodies.
Solving problems and validating assays regarding IEF/SDS capillary
electrophoresis. Glycan structure analysis, optimizing the peptide
mapping of the Monoclonal antibodies. Involvement in PLA compilation
and submission to ICH (EMEA/FDA) standards. Designed and performed
various method validations for a number of techniques. Performance
verification and operational qualification of various equipment.
Experience in GLP, GMP, and SLP working practices
The Ph.D. project involved collaboration between the University
of London and the Institute of Food Research at Reading University.
The overall aim of the project was to investigate the thermo
stability and the pH stability of Chymosin and other aspartic
proteinase enzymes; this was involving both molecular biology
and biochemical investigation through a fungal expression system.
In addition, a variety of specific mutations have been introduced
into the Chymosin gene.
The objective of my postdoctoral work is to have a clear idea
regarding the structure of the hERaLBD. Currently, Dr. Zacharewski
employs studies that are able to monitor the estrogenic potency
of chemicals by their ability to bind the human estrogen receptor
(hER) and cause upregulation of an engineered reporter gene
construct. These studies are very specific for monitoring chemicals
that will bind the human receptor. The study involve expression,
purification and crystallization of the hERaLBD. The work also
involves investigation of the effect of gestational and lactational
exposure to estrogenic chemicals (i.e. estrogenic endocrine
disrupters) on testicular gene expression and spermatogenesis
in mice. This project test the hypothesis that exposure to endocrine
disrupters during development compromises male fertility as
a result of alterations in testicular gene expression profiles,
sperm assessment. This investigated through in vitro fertilization,
in situ hybridization, cDNA array technology, bioinformatics
and the statistical analysis of gene expression data.
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