Biochemistry 472 Learning Modules
- Periods 2A and 2B: Growth of bacteria monitored by spectrophotometry and plating.
- Periods 3 and 4: Growth of bacteriophage LambdaZAPII/topAcysB.
- Periods 5 and 6: Preparation of bacteriophage and plasmid DNA.
- Period 7 : Electrophoretic Analysis of DNA preps.
- Period 8 : Directional cloning of topAcysB into pTrc99A.
- Period 9B : Electrophoretic Analysis of Restricted DNAs and Products of Ligation
- Period 10 : Transformation of DM700 and XL-1 Blue with pTrc99A/topAcysB
- Period 16 : Analysis of Topoisomerase I Assays
- Period 20 : Densitometric Analysis of SDS-PAGE Gels and Western Blots.
- Period 21A : PCR Amplification of DNA and EcoRI Digestion
- Period 22 : Ligation of topA DNA into M13mp19
- Period 23 : Transfection of F' E. coli cells with the Ligation Mixture of topA DNA Inserted into M13mp19
- Period 25 : Orientation Analysis of topA Subclones by a Complimentarity Test
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