Biochemistry 472 Learning Modules

• Periods 2A and 2B: Growth of bacteria monitored by spectrophotometry and plating.
• Periods 3 and 4: Growth of bacteriophage LambdaZAPII/topAcysB.
• Periods 5 and 6: Preparation of bacteriophage and plasmid DNA.
• Period 7 : Electrophoretic Analysis of DNA preps.
• Period 8 : Directional cloning of topAcysB into pTrc99A.
• Period 9B : Electrophoretic Analysis of Restricted DNAs and Products of Ligation
• Period 10 : Transformation of DM700 and XL-1 Blue with pTrc99A/topAcysB
• Period 16 : Analysis of Topoisomerase I Assays
• Period 20 : Densitometric Analysis of SDS-PAGE Gels and Western Blots.
• Period 21A : PCR Amplification of DNA and EcoRI Digestion
• Period 22 : Ligation of topA DNA into M13mp19
• Period 23 : Transfection of F' E. coli cells with the Ligation Mixture of topA DNA Inserted into M13mp19
• Period 25 : Orientation Analysis of topA Subclones by a Complimentarity Test

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