RTSF Flow Cytometry Core Facility

Operator:
Dr. Louis King
Ph:355-1536
419 Biochemistry
LSRII - 5155 BPS
kingl@msu.edu

Hours:

Available daily by prior appointment

Rates:

• FACS Analysis - $20/hr, 1 hr minimum
• Fixed Cell Sorting - $20 setup plus $20/hr sort time used
• Viable Cell Sorting - $40 setup plus $20/hr sort time used
• PC Workstation, software, and printing $20/hr
• Consulting on experimental design and data analysis- $20/hr

(See data sample.)

MSU's Flow Cytometry facility is equipped with two flow cytometers:

1) BD Vantage SE turbo sort with DIVA electronics + software
    (See system configuration.)

2) LSRII digital flow cytometer with DIVA software

Both flow cytometers are multiple laser based optical instruments that analyze cell populations for multiple characteristics simultaneously.
(See data sample.)

Analysis

• Cell cycle analysis and ploidy
• Apotosis
• Mitochondrial activity
• Ca++ release
• Changes in the plasma membrane.
• Expression of 1-8 cell surface proteins, receptors, or adhesion molecules.
• Expression of cytoplasmic enzymes, hormone receptors, and transport proteins.
• Detection of nuclear proteins, oncogenes, and growth factors.

Combinatorial Analysis

FACS can rapidly assess individual cells for multiple biochemical parameters. It can combine DNA analysis with quantitation of cell surface markers to observe changes in expression of receptors, CD markers, enzymes, changes in cell cycle status, etc.

Cell Sorting

FACS can select and purify cells in a particular phase of the cell cycle, cells expressing high levels of a receptor, cells devoid of a particular phosphatase or oncogene, etc. FACS can also identify and sort for specific mutants or select for positive transfected cells.