UDP/dTDP-D-glucosyltranferase
GtfB from
the
chloroeremomycin biosynthetic pathway
|
A stereo ribbon drawing of UDP/dTDP-D-glucosyltranferase GtfB from the chloroeremomycin biosynthetic pathway. This family of bi-domain enzymes is constructed of two Rossmann folds (with the beta sheets shown in blue). The N-terminal domain (bottom) binds the vancomycin heptapeptide aglycone, while the C-terminal domain binds UDP-glucose. GtfB transfers the glucose from UDP-glucose to a hydroxyl on the 4th residue of the vancomycin heptapeptide aglycone. |
dTDP-L- epi-vancosamsyltranferase GtfA from
the chloroeremomycin biosynthetic pathway
|
A stereo ribbon drawing of dTDP-L-epi-vancosaminyltranferase GtfA from the
chloroeremomycin biosynthetic pathway; the beta sheets of the two Rossmann
folds are shown in green. The
N-terminal domain has the monoglucosylated heptapeptide acceptor bound, while
the C-terminal domain has bound dTDP.
GtfA transfers L-epi-vancosamine
from dTDP-L-epi-vancosamine to
a hydroxyl on the 6th residue of the vancomycin heptapeptide
aglycone. |
dTDP-L-vancosaminyltranferase GtfD from
the vancomycin biosynthetic pathway
|
A stereo ribbon drawing of
dTDP-L-vancosaminyltranferase GtfD from the vancomycin biosynthetic pathway;
the beta sheets of the two Rossmann folds are shown in magenta. The N-terminal domain has the monoglucosylated
heptapeptide acceptor bound, while the C-terminal domain has bound dTDP. GtfD transfers L-vancosamine from
dTDP-L-vancosamine to the 2’ hydroxyl on the glucose of the monoglucosylated
heptapeptide acceptor. |
|
References of interest Mulichak, A. M., Losey, H. C., Walsh, C. T., and Garavito, R. M. (2001)
Structure of the UDP-glucosyltransferase GtfB that Modifies the Heptapeptide
Aglycone in Biosynthesis of the Vancomycin Group of Antibiotics. Structure 9,
547-57. Mulichak, A. M., Losey, H. C., Lu, W., Walsh, C. T., and Garavito, R. M.
(2003) Structure of the TDP-epi-Vancosaminyltransferase
GtfA from the Chloroeremomycin Biosynthetic Pathway. Proc. Natl. Acad. Sci.
USA 100, 9238-43. Mulichak, A. M., W. Lu, H. C. Losey, C. T. Walsh, and R. M. Garavito (2004) “Crystal Structure of
Vancosaminyltransferase GtfD from the Vancomycin Biosynthetic Pathway:
Interactions with Acceptor and Nucleotide Ligands.” Biochemistry, 43,
5170-80. |