R. Michael Garavito Recent Publications

Lin, H. J., Levine, A. J., Materi, A. M., Park, J. M., Patterson, R. E., Goodmand, J. E., Chlebowski, R. T., Sansbury, L. B., Kekug, T. O., Henderson, B. E., Kolonel, L. N., Le Marchand, L., Harris, C. C., Sandler, R. S., Haile, R. W., Garavito, R. M., Lawson, J. A., and Kapoor, S. (2007) "Prostaglandin H synthase 2 (PTGS2, or Cox-2) Val511Ala variant in African Americans: pooled analyses of four case-control studies of colorectal cancer and measurements of prostaglandin synthesis and excretion in vivo." Prostaglandins, Leukotrienes and Essential Fatty Acids, in press.

Wada M, DeLong CJ, Hong YH, Rieke CJ, Song I, Sidhu RS, Yuan C, Warnock M, Schmaier AH, Yokoyama C, Smyth EM, Wilson SJ, FitzGerald GA, Garavito RM, Sui de X, Regan JW, Smith WL. (2007) Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. J Biol Chem. 3;282(31):22254-66.

Harman CA, Turman MV, Kozak KR, Marnett LJ, Smith WL, Garavito RM. (2007) Structural basis of enantioselective inhibition of cyclooxygenase-1 by S-alpha-substituted indomethacin ethanolamides. J Biol Chem. 21;282(38):28096-105.

Qin L, Sharpe MA, Garavito RM, Ferguson-Miller S. (2007) Conserved lipid-binding sites in membrane proteins: a focus on cytochrome c oxidase. Curr Opin Struct Biol. 17(4):444-50.

Qin L, Mills DA, Hiser C, Murphree A, Garavito RM, Ferguson-Miller S, Hosler J. (2007) Crystallographic location and mutational analysis of Zn and Cd inhibitory sites and role of lipidic carboxylates in rescuing proton path mutants in cytochrome c oxidase. Biochemistry. 29;46(21):6239-48.

Qin, L., C. Hiser, A. M. Mulichak, R. M. Garavito, and S. Ferguson-Miller. (2006) Crystal Structure at 2.0 Å Resolution of the Catalytic Core of Cytochrome c Oxidase and Identification of Conserved Lipid Binding Sites. Proc. Natl. Acad. Sci. 103, 16117-22.

Shimojima M, Hoffmann-Benning S, Garavito RM, Benning C. (2005) Ferredoxin-dependent glutamate synthase moonlights in plant sulfolipid biosynthesis by forming a complex with SQD1. Arch Biochem Biophys. Apr 1;436(1):206-14.

Barbier G, Oesterhelt C, Larson MD, Halgren RG, Wilkerson C, Garavito RM, Benning C, Weber AP. (2005) Comparative genomics of two closely related unicellular thermo-acidophilic red algae, Galdieria sulphuraria and Cyanidioschyzon merolae, reveals the molecular basis of the metabolic flexibility of Galdieria sulphuraria and significant differences in carbohydrate metabolism of both algae. Plant Physiol. Feb;137(2):460-74.

Weber AP, Oesterhelt C, Gross W, Brautigam A, Imboden LA, Krassovskaya I, Linka N, Truchina J, Schneidereit J, Voll H, Voll LM, Zimmermann M, Jamai A, Riekhof WR, Yu B, Garavito RM, Benning C. (2004) EST-analysis of the thermo-acidophilic red microalga Galdieria sulphuraria reveals potential for lipid A biosynthesis and unveils the pathway of carbon export from rhodoplasts. Plant Mol Biol. May;55(1):17-32.

Harman CA, Rieke CJ, Garavito RM, Smith WL. (2004) Crystal structure of arachidonic acid bound to a mutant of prostaglandin endoperoxide H synthase-1 that forms predominantly 11-hydroperoxyeicosatetraenoic acid. J Biol Chem. Oct 8;279(41):42929-35. Epub 2004 Jul 30.

Mulichak AM, Lu W, Losey HC, Walsh CT, Garavito RM. (2004) Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands. Biochemistry. May 11;43(18):5170-80.

Webb NA, Mulichak AM, Lam JS, Rocchetta HL, Garavito RM. (2004) Crystal structure of a tetrameric GDP-D-mannose 4,6-dehydratase from a bacterial GDP-D-rhamnose biosynthetic pathway. Protein Sci. Feb;13(2):529-39.

Mulichak AM, Losey HC, Lu W, Wawrzak Z, Walsh CT, Garavito RM. (2003) Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway. Proc Natl Acad Sci U S A. Aug 5;100(16):9238-43. Epub 2003 Jul 21.

Garavito RM, Mulichak AM. (2003) The structure of mammalian cyclooxygenases. Annu Rev Biophys Biomol Struct.;32:183-206. Epub 2003 Feb 5. Review.

Mulichak AM, Bonin CP, Reiter WD, Garavito RM. (2002) Structure of the MUR1 GDP-mannose 4,6-dehydratase from Arabidopsis thaliana: implications for ligand binding and specificity. Biochemistry. Dec 31;41(52):15578-89.

Lin HJ, Lakkides KM, Keku TO, Reddy ST, Louie AD, Kau IH, Zhou H, Gim JS, Ma HL, Matthies CF, Dai A, Huang HF, Materi AM, Lin JH, Frankl HD, Lee ER, Hardy SI, Herschman HR, Henderson BE, Kolonel LN, Le Marchand L, Garavito RM, Sandler RS, Haile RW, Smith WL. (2002) Prostaglandin H synthase 2 variant (Val511Ala) in African Americans may reduce the risk for colorectal neoplasia. Cancer Epidemiol Biomarkers Prev. Nov;11(11):1305-15.

Garavito RM, Malkowski MG, DeWitt DL. (2002) The structures of prostaglandin endoperoxide H synthases-1 and -2. Prostaglandins Other Lipid Mediat. Aug;68-69:129-52. Review.

Thuresson, E.D., Lakkides, K. M., Rieke, C.J., Sun, Y., Wingerd, B., Micielli, R. J., Mulichak, A. M., Malkowski, M. G., Garavito, R. M., Smith, W.L. (2001) Prostaglandin endoperoxide synthase-1: The functions of cycloxygenase active site residues in the binding, positioning and oxygenastion of arachidonic acid. J. Biol. Chem. 276, 10347-59.

Thuresson, E.D,. Malkowski, M. G., Lakkides, K. M., Rieke, C.J., Mulichak, A. M. , Ginell, S., Garavito, R. M., Smith, W.L. (2001) Mutational and X-ray crystallographic analysis of the interaction of homo-gamma-linolenic acid with prostaglandin endoperoxide synthases. J. Biol. Chem. 276, 10358-65.

Sanda, S., Leustek, T., Theisen, M. J., Garavito, R. M., and Benning, C. (2001) Recombinant Arabidopsis SQD1 converts UDP-glucose and sulfite to the sulfolipid headgroup precursor UDP-sulfoquinovose. J. Biol. Chem. 276, 3941-3946.

Mulichak, A. M., Losey, H. C., Walsh, C. T., and Garavito, R. M. (2001) Structure of the UDP-glucosyltransferase GtfB that Modifies the Heptapeptide Aglycone in Biosynthesis of the Vancomycin Group of Antibiotics. Structure 9, 547-557.

M. G. Malkowski, E. D. Thuresson, K. M. Lakkides, C. J. Rieke, R. Micielli, W. L. Smith, and R. M. Garavito (2001) Structure of Eicosapentaenoic and Linoleic acids in the cyclooxygenase site of prostaglandin endoperoxide H synthase-1. J. Biol. Chem. 276, 37547-37555.

Garavito, R. M., and Ferguson-Miller, S. (2001) Detergents as Tools in Membrane Biochemistry. J. Biol. Chem. 276, 32403-6.

Garavito, R. M. (2001) Prostaglandin endoperoxide H synthase-1 and -2. In Handbook of Metalloproteins (K. Wieghardt, R. Huber, T. L. Poulos, and A. Messerschmidt, eds.). John Wiley, Sussex, Vol. 2, 245-63.

Schelvis, J. P. M.,Seibold, S.A., Cerda, J.F., Garavito, R. M., Arakawa, T., and Babcock, G.T. (2000). Interaction of nitric oxide with prostaglandin endoperoxide H synthase: Implications for Fe-His bond cleavage in heme proteins. J. Phys. Chem. B 104, 10844-10850.

Malkowski, M.G., Ginell, S., Smith, W.L., and Garavito, R. M. (2000) Structure of prostaglandin synthase complexed with arachidonic acid. Science 289,1933-37.

Garavito home page