Robert P. Hausinger Recent Publications

Li M, Müller TA, Fraser BA, Hausinger RP. 2008. Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans. Arch Biochem Biophys. 470:44-53.

Grzyska PK, Hausinger RP. 2007. Cr(II) reactivity of taurine/alpha-ketoglutarate dioxygenase. Inorg Chem. 46:10087-10092.

Grossoehme NE, Mulrooney SB, Hausinger RP, Wilcox DE. Thermodynamics of Ni2+, Cu2+, and Zn2+ binding to the urease metallochaperone UreE. Biochemistry. 2007 Sep 18;46(37):10506-16.

Hausinger, R. P. and D. B. Zamble. 2007. Microbial physiology of nickel and cobalt. Molecular Microbiology of Heavy Metals (ed. Nies, D. H. and S. Silver) Springer, Heidelberg, Germany 6:287-320.

Muthukumaran, R. B., P. K. Grzyska, R. P. Hausinger, and J. McCracken. 2007. Probing the Fe-substrate orientation for taurine/a-ketoglutarate dioxygenase using deuterium ESEEM spectroscopy. Biochemistry 46 (20): 5951-5959.

Grzyska, P. K., T. A. Müller, M. G. Campbell, and R. P. Hausinger. 2007. Metal ligand substitution and evidence for quinone formation in taurine/a-ketoglutarate dioxygenase. J. Inorg. Biochem. 101:797-808.

Yu, Z., P.-A. Genest, B. ter Riet, K. Sweeney, C. DiPaolo, R. Kieft, E. Christodoulou, A. Perrakis, J. M. Simmons, R. P. Hausinger, H. G. A. M. van Luenen, D. J. Rigden, R. Sabatini, and P. Borst. 2007. The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase. Nucl. Acids Res. 35 (7): 2107-2115.

Montero-Morán, G. M., M. Li, E. Rendòn-Huerta, F. Jourdan, D. J. Lowe, A. W. Stumpff-Kane, M. Feig, C. Scazzocchio, and R. P. Hausinger. 2007. Purification and characterization of the FeII- and a-ketoglutarate-dependent Aspergillus nidulans xanthine hydroxylase expressed in A. nidulans and Escherichia coli. Biochemistry 46 (18): 5293-5304.

Quiroz, S., J. K. Kim, S. B. Mulrooney, and R. P. Hausinger. 2007. Chaperones of nickel metabolism. In Nickel and Its Surprising Impact on Nature, Vol. 2 of Metal Ions in Life Sciences (ed. Sigel, A, H. Sidel, R. K. O. Sigel) John Wiley & Sons, Ltd., Chichester, UK, pp. 519-544.

Hausinger, R. P. 2007. New insights into acetone metabolism. J. Bacteriol. 189:671-673.

Hausinger, R. P., and A. T. Phillips. 2007. Enzyme activity. In: Methods in General and Molecular Microbiology (ed. C. A. Reddy) p.504-526.

Kim, J. K., S. B. Mulrooney, and R. P. Hausinger. 2006. The UreEF fusion protein provides a soluble and functional form of the UreE urease accessory protein. J. Bacteriol. 188:8413-8420.

Müller, T. A., M. I. Zavodszky, M. Feig, L. A. Kuhn, and R. P. Hausinger. 2006. Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/a-ketoglutarate dioxygenases. Protein Sci. 15:1356-1368.

Luo, L., M. B. Pappalardi, P. J. Tummino, R. A. Copeland, M. E. Fraser, P. K. Grzyska, and R. P. Hausinger. 2006. An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation. Anal. Biochem. 353:69-74.

Zhang, L., S. B. Mulrooney, A. F. K. Leung, Y. Zeng, B. B. C. Ko, R. P. Hausinger, and H. Sun. 2006. Inhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugs. BioMetals 19:503-511.

Rohankhedkar, M., Mulrooney, S. B., Wedemeyer, W. J., Hausinger, R. P. 2006. The AidB component of the Escherichia coli adaptive response to alkylating agents is a flavin-containing, DNA-binding protein. J. Bacteriol. 188:223-230.

Koehntop, K. D., Marimanikkuppam, S., Ryle, M. J., Hausinger, R. P., Que, L., Jr. 2006. Self-hydroxylation of taurine/a-ketoglutarate dioxygenase. Evidence for more than one oxygen activation mechanism. J. Biol. Inorg. Chem. 11:63-72.

Mulrooney, S. B., and R. P. Hausinger. 2005. Metallocenter biosynthesis and assembly. Encyclopedia of Inorganic Chemistry, 2nd Edition. R. B. King, Ed., John Wiley & Sons, Chichester, UK, pp.3144-3152.

Kalliri E, Grzyska PK, Hausinger RP. 2005. Kinetic and spectroscopic investigation of Co(II), Ni(II), and N-oxalylglycine inhibition of the Fe(II)/alpha-ketoglutarate dioxygenase, TauD. Biochem Biophys Res Commun. Dec 9;338(1):191-7.

Kim, J. K., Mulrooney, S. B., and Hausinger, R. P. 2005. Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins. J. Bacteriol. 187:7150-7154.

Grzyska, P. K., M. J. Ryle, G. R. Monterosso, J. Liu, D. P. Ballou, and R. P. Hausinger. 2005. Steady-state and transient kinetic analyses of taurine/a-ketoglutarate dioxygenase: effects of oxygen concentration, alternative sulfonates, and active site variants on the Fe(IV)-oxo intermediate. Biochemistry 44:3845-3855.

Mulrooney, S. B., S. K. Ward, and R. P. Hausinger. 2005. Purification and properties of the Klebsiella aerogenes UreE metal-binding domain, a functional metallochaperone of urease. J. Bacteriol. 187 (10): 3581-3585.

Kuchar, J., and R. P. Hausinger. 2004. Biosynthesis of Metal Sites. Chem. Rev. 104:509-526.

Henshaw, T. F., M. Feig, and R. P. Hausinger. 2004. Aberrant activity of the DNA repair enzyme AlkB. J. Inorg. Biochem. 98:856-861.

Proshlyakov, D. A., T. F. Henshaw, G. R. Monterosso, M. J. Ryle, and R. P. Hausinger. 2004. Direct detection of oxygen intermediates in the non-heme Fe enzyme taurine/a-ketoglutarate dioxygenase. J. Am. Chem. Soc. 126:1022-1023.

Hausinger, R. P. 2004. Fe(II)/a-ketoglutarate-dependent hydroxylases and related enzymes. Crit. Rev. Biochem. Molec. Biol. 39:21-68.

Chang, Z., J. Kuchar, and R. P. Hausinger. 2004. Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease. J. Biol. Chem. 279:15305-15313.

Hausinger, R. P. 2004. Metabolic versatility of prokaryotes for urea decomposition. J. Bacteriol. 186:2520-2522.

Mulrooney, S. B., and R. P. Hausinger. 2003. Metal ion dependence of recombinant Escherichia coli allantoinase. J. Bacteriol., 185:126-134.

Ryle, M. J., A. Liu, R. B. Muthukumaran, R. Y. N. Ho, K. D. Koehntop, J. McCracken, L. Que, Jr., and R. P. Hausinger. 2003. O2- and alpha-ketoglutarate-dependent tyrosyl radical formation in TauD, an alpha-keto acid-dependent non-heme iron dioxygenase. Biochemistry 42:1854-1862.

Mulrooney, S. B., and R. P. Hausinger. 2003. Nickel uptake and utilization by microorganisms. FEMS Microbiol. Rev. 27:239-261.

Ryle, M. J., K. D. Koehntop, A. Liu, L. Que, Jr., and R. P. Hausinger. 2003. Interconversion of two oxidized forms of taurine/alpha-ketoglutarate dioxygenase, a non-heme iron hydroxylase: evidence for bicarbonate binding. Proc. Natl. Acad. Sci. USA 100:3790-3795.

Dunning Hotopp, J. C., T. A. Auchtung, D. A. Hogan, and R. P. Hausinger. 2003. Intrinsic tryptophan fluorescence as a probe of metal and a-ketoglutarate binding to TfdA, a mononuclear non-heme iron oxygenase. J. Inorg. Biochem. 93:66-70

Hausinger, R. P. 2003. Ni and CO: more surprises. Nat. Struct. Biol. 10:234-236.

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