A. Daniel Jones
My research interests lie in improving mass spectrometry and separation strategies and applying them to perform global profiling of metabolites. This approach, known as metabolomics, probes the influence of genetics and environment on rates of biosynthesis and degradation of metabolites. Such measurements lie at the heart of systems biology approaches for engineering plants and microorganisms for improved productivity, as biosensors, and as valuable sources of an assortment of bioactive chemicals. Furthermore, the information in the metabolome can be used as biomarkers of stress, toxicity, and disease.
The diversity of metabolites produced by plants, fungi, and bacteria has been minimally explored, yet many of these compounds have potent biological activities that influence interactions involving plants, microbes, and insects. In several interdisciplinary collaborations, my research group has developed sensitive analytical schemes for characterizing, quantifying, and sorting these unknown metabolites according to chemical classes based on information contained in their mass spectra.
Our ongoing efforts are exploring the boundaries of fast-scanning high-resolution time-of-flight mass spectrometry and other MS/MS approaches to improve characterization of the metabolome using an assortment of plant and animal model systems. Plants appear to distinguish different modes of wounding (e.g. mechanical wounding from insect herbivory and pathogen infections), and their chemistry changes in distinct ways as a response to different environmental stresses. Precursor ion MS/MS scans have shown that different insect species deposit different phospholipids profiles onto plants while feeding, and these lipids may play important signaling roles that allow plants to distinguish stresses and respond with different defensive chemicals.
More targeted analyses of electrophilic signaling lipids are achieved through chemical derivatization that can be used for selective metabolite enrichment. Our group is also developing chiral monolithic capillary columns for sensitive HPLC and capillary electrochromatographic separations of metabolite enantiomers that will allow for improved metabolite localization and analysis of less abundant metabolites.
Schmidt A, Li C, Jones AD, Pichersky E. 2012. Characterization of a flavonol 3-O-methyltransferase in the trichomes of the wild tomato species Solanum habrochaites. Planta 236:839-849.
Angrish MM, Mets BD, Jones AD, Zacharewski TR. 2012. Dietary fat is a lipid source in 2,3,7,8-Tetrachlorodibenzo-rho-Dioxin (TCDD)-elicited hepatic steatosis in C57BL/6 Mice. Toxicological Sciences 128:377-386
Pham NT, Jewell WT, Morin D, Jones AD, Buckpitt AR. 2012. Characterization of model peptide adducts with reactive metabolites of naphthalene by mass spectrometry. PLoS One 7(8):e42053.
Shafii B, Vismeh R, Beaudry R, Warner R, Jones AD. 2012. Large-scale profiling of diterpenoid glycosides from Stevia rebaudiana using ultrahigh performance liquid chromatography/tandem mass spectrometry. Anal Bioanal Chem. 403(9):2683-90
Lau MW, Bals BD, Chundawat SPS, Jin MJ, Gunawan C, Balan V, Jones AD, Dale, BE. 2012. An integrated paradigm for cellulosic biorefineries: utilization of lignocellulosic biomass as self-sufficient feedstocks for fuel, food precursors and saccharolytic enzyme production Energy & Environmental Science 5(5):7100-7110.
Hao JJ, Liu HW, Donis-Gonzalez IR, Lu XH, Jones AD, Fulbright DW. 2012. Antimicrobial activity of chestnut extracts for potential use in managing soilborne plant pathogens. Plant Disease 96(3):354-360.
Lu J, Jones AD, Harkema JR, Roth RA, Ganey PE. 2012. Amiodarone exposure during modest inflammation induces idiosyncrasy-like liver injury in rats: role of tumor necrosis factor-alpha. Toxicol Sci. 125(1):126-33.
Gu L, Jones AD, Last RL. 2012. Rapid LC-MS/MS profiling of protein amino acids and metabolically related compounds for large-scale assessment of metabolic phenotypes. Methods Mol Biol. 828:1-11.
Angrish MM, Jones AD, Harkema JR, Zacharewski TR. 2011. Aryl hydrocarbon receptor-mediated induction of Stearoyl-CoA desaturase 1 alters hepatic fatty acid composition in TCDD-elicited steatosis. Toxicol Sci. 124(2):299-310.
Varbanova M, Porter K, Lu F, Ralph J, Hammerschmidt R, Jones AD, Day B. 2011. Molecular and biochemical basis for stress-induced accumulation of free and bound p-coumaraldehyde in cucumber. Plant Physiol. 157(3):1056-66.
Nobuko S, Jones AD, Randolph B. 2011. Changes in free amino acid content in 'Jonagold' apple fruit as related to branched-chain ester production, ripening, and senescence. J of the Am Soc for Hort Sci 136 (6):429-440
Zeng W, Brutus A, Kremer JM, Withers JC, Gao X, Jones AD, He SY. 2011. A genetic screen reveals Arabidopsis stomatal and/or apoplastic defenses against Pseudomonas syringae pv. tomato DC3000. PLoS Pathog. 7(10):e1002291.
Xi X, Johnson NS, Brant CO, Yun SS, Chambers KL, Jones AD, Li W. 2011. Quantification of a male sea lamprey pheromone in tributaries of Laurentian Great Lakes by liquid chromatography-tandem mass spectrometry. Environ Sci Technol. 45(15):6437-43.MORE