Claire Vieille
Research Associate Professor
Co-Director - QBI
- B.S. 1986, Institut National Agronomique Paris-Grignon, Paris, France
- M.S 1985, Université Paris 7 at Institut Pasteur, Paris, France
- PhD 1991, Université Paris 7 at Institut Pasteur, Paris, France
Office: 110A Biochemistry Bldg (Phone: 517-355-9722)
Lab: 110 Biochemistry Bldg
(Phone: 517-353-4674)
Michigan State University
East Lansing, MI 48824-1319
Fax: 517-353-9334
Quantitative Biology Initiative
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Claire Vieille
Research Interests
Research in my lab focuses on biotechnology, more particularly on the design and development of industrial biocatalysts. Among the many industrial applications of bioprocesses are the use of microbial and enzyme biocatalysts in biochemical and biomaterial production, drug manufacture, sensors and diagnostics, and food and feed production. Our research on enzyme biocatalysts focuses primarily on enzymes from thermophiles and hyperthermophiles. With their high thermostability and solvent tolerance, these enzymes are attractive candidates for industrial processes, which typically require catalysts that are robust, highly active, and have high chemical yields. These enzymes are also ideal model proteins to study the mechanisms underlying protein thermostability. Our work on microbial catalysts focuses on organic acid (succinate and aspartate) production by a natural high-succinate producing bacterial species.
Enzyme engineering. Our work on enzymes from thermophiles and hyperthermophiles focuses on six enzymes: 1) adenylate kinase, a small protein used to study how flexibility relates to stability and activity; 2) xylose (glucose) isomerase used in high fructose corn syrup manufacture; 3) secondary alcohol dehydrogenase (SADH) used in chiral chemical synthesis;
4) alkaline phosphatase used in diagnostics; 5) aldose reductase, to be used to produce sorbitol from glucose; and 6) mannitol dehydrogenase to be used in combination with xylose isomerase for mannitol production from glucose.
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Recent Publications
Musa, M.M., K.I. Ziegelmann-Fjeld, C. Vieille, and R.S. Phillips. 2008. Activity and selectivity of W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus in organic solvents and ionic liquids: Mono- and biphasic media. Org. Biomol. Chem. DOI: 10.1039/b717120j. Link to publication
McKinlay, J.B., and C. Vieille. 2008. 13C-metabolic flux analysis of Actinobacillus succinogenes fermentative metabolism at different NaHCO3 and H2 concentrations. Metab. Engin. 10:55–68. Link to publication
McKinlay, J.B., C. Vieille, and J.G. Zeikus. 2007. Prospects for a bio-based succinate industry. Appl. Microbiol. Biotechnol. 76:727–740. Link to publication
Puttick, P., C. Vieille, S.H. Song, M.N. Fodje, P. Grochulski and L.T.J. Delbaere. 2007. Crystallization, preliminary X-ray diffraction and structure analysis of Thermotoga maritima mannitol dehydrogenase. Acta Crystallog. Section F 63: 350–352. Link to publication
McKinlay JB, Y Shachar-Hill, JG Zeikus , and C Vieille. 2007. Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analyses of 13C-labeled metabolic product isotopomers. Metab. Engin. 9:177–192. Link to publication
Musa MM, KI Ziegelmann-Fjeld, C Vieille, JG Zeikus, and RS Phillips. 2007. Asymmetric reduction and enantioselective oxidation using W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus. J. Org. Chem. 72:30–34. Link to publication
Musa MM, KI Ziegelmann-Fjeld, C Vieille, JG Zeikus, and RS Phillips. 2007. Sol-gel encapsulated W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus performs asymmetric reduction of hydrophobic ketones in organic solvent. Angewandte Chemie. 46:3091–3094. Link to publication
Ziegelmann-Fjeld, KI, MM Musa, RS Phillips, JG Zeikus, and C Vieille. 2007. Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase mutant highly active and stereoselective on phenylacetone and benzylacetone. Protein Engin. Des. Select. 20:47–55. Link to publication