Claire Vieille
Research Interests
Research in my lab focuses on biotechnology, more particularly on the design and development of industrial biocatalysts. Among the many industrial applications of bioprocesses are the use of microbial and enzyme biocatalysts in biochemical and biomaterial production, drug manufacture, sensors and diagnostics, and food and feed production. Our research on enzyme biocatalysts focuses primarily on enzymes from thermophiles and hyperthermophiles. With their high thermostability and solvent tolerance, these enzymes are attractive candidates for industrial processes, which typically require catalysts that are robust, highly active, and have high chemical yields. These enzymes are also ideal model proteins to study the mechanisms underlying protein thermostability. Our work on microbial catalysts focuses on organic acid (succinate and aspartate) production by a natural high-succinate producing bacterial species.
Recent Publications
Song SH, Vieille C. 2009. Recent advances in the biological production of mannitol. Appl Microbiol Biotechnol. 84(1):55-62. Link to publication
Krishnamurthy, H., K. Munro, H. Yan, and C. Vieille. 2009. Dynamics in T. neapolitana adenylate kinase. 15N relaxation and hydrogen-deuterium exchange studies of a hyperthermophilic enzyme highly active at 30°C. Biochemistry. 48:2723-2739. Link to publication
Song SH, Ahluwalia N, Leduc Y, Delbaere LTJ, Vieille C. 2008. Thermotoga maritima TM0298 is a highly thermostable mannitol dehydrogenase. Applied Microbiology and Biotechnology. 81: 485-495. Link to publication
Musa, M.M., K.I. Ziegelmann-Fjeld, C. Vieille, and R.S. Phillips. 2008. Activity and selectivity of W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus in organic solvents and ionic liquids: Mono- and biphasic media. Org. Biomol. Chem. DOI: 10.1039/b717120j. Link to publication
McKinlay, J.B., and C. Vieille. 2008. 13C-metabolic flux analysis of Actinobacillus succinogenes fermentative metabolism at different NaHCO3 and H2 concentrations. Metab. Engin. 10:55-68. Link to publication
McKinlay, J.B., C. Vieille, and J.G. Zeikus. 2007. Prospects for a bio-based succinate industry. Appl. Microbiol. Biotechnol. 76:727-740. Link to publication
Puttick, P., C. Vieille, S.H. Song, M.N. Fodje, P. Grochulski and L.T.J. Delbaere. 2007. Crystallization, preliminary X-ray diffraction and structure analysis of Thermotoga maritima mannitol dehydrogenase. Acta Crystallog. Section F 63: 350-352. Link to publication
McKinlay JB, Y Shachar-Hill, JG Zeikus , and C Vieille. 2007. Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analyses of 13C-labeled metabolic product isotopomers. Metab. Engin. 9:177-192. Link to publication
Musa MM, KI Ziegelmann-Fjeld, C Vieille, JG Zeikus, and RS Phillips. 2007. Asymmetric reduction and enantioselective oxidation using W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus. J. Org. Chem. 72:30-34. Link to publication
Musa MM, KI Ziegelmann-Fjeld, C Vieille, JG Zeikus, and RS Phillips. 2007. Sol-gel encapsulated W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus performs asymmetric reduction of hydrophobic ketones in organic solvent. Angewandte Chemie. 46:3091-3094. Link to publication
Ziegelmann-Fjeld, KI, MM Musa, RS Phillips, JG Zeikus, and C Vieille. 2007. Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase mutant highly active and stereoselective on phenylacetone and benzylacetone. Protein Engin. Des. Select. 20:47-55. Link to publication