Jack Throck Watson Recent Publications

Watson, JT and Sparkman, OD (2007) Introduction to Mass Spectrometry: Instrumentation, Applications, and Strategies for Data Interpretation 4th Edition, 818pp, J. Wiley & Sons, Chichester, England ISBN 780470516348

Dunn JD, Watson JT, Bruening ML. (2006) Detection of phosphopeptides using Fe(III)-nitrilotriacetate complexes immobilized on a MALDI plate Analytical Chemistry 78 (5): 1574-1580.

Gallegos-Perez JL, Rangel-Ordonez L, Bowman SR, et al. (2005) Study of primary amines for nucleophilic cleavage of cyanylated cystinyl proteins in disulfide mass mapping methodology Analytical Biochemistry 346 (2): 311-319.

Li, Xue; Hood, Robin J.; Wedemeyer, William J. and Watson, J. Throck. (2005) Characterization of Peptide Folding Nuclei by Hydrogen/Deuterium Exchange-Mass Spectrometry. Protein Science 14:1922-1928.

Wu, W., Huang, W., Qi,,J., Torng, E., and Watson. J.T. (2004) Analysis of 'Signature Sets' and their Application in Unambiguous Identification of Disulfide Structure in Proteins by Mass-Mapping Structure-Related Fragments. J. Proteomic Research 3(4):770-777.

Li, X., Chou, Y.-T., Husain, Rhonda, and Watson, J. T. (2004) Integration of Hydrogen/Deuterium Exchange and Cyanylation-Based Methodology for Conformational Studies of Cystinyl Proteins, Anal. Biochem. 331:130-137.

Xu,Y., Bruening, M., and Watson, J.T. (2004) Use of Polymer-Modified MALDI-MS Probes to Improve Analyses of Protein Digests and DNA. Anal Chem. 76(11):3106-11.

Zhang, H., Wu, W., Du Y., Santos S.J., Conrad S.E., Watson J.T., Grammatikakis N. and Gallo, K. (2004) Hsp90/ p50cdc37 Is Required for Mixed Lineage Kinase 3 Signaling. J.Biol.Chem. 279:19457-63.

Borges, C. R., Qi, J., Wu, W., Torng, E., Hinck, A. P., and Watson, J. T. (2004) Algorithm-Assisted Elucidation of Disulfide Structure: Application of the Negative Signature Mass Algorithm to Mass-Mapping the Disulfide Structure of the 12-Cysteine Transforming Growth Factor ß Type II Receptor Extracellular Domain. Analytical Biochemistry 329(1) 91-103.

Wu, W., Watson, J. T., Stevens, F. J., Yousefzai, R., and Anderson, L. E. (2004) CN-Induced Cleavage and Mass Mapping Indicates that Chloroplast Fructose Bisphosphatase Contains Alternate Disulfide Bonds. Photosynthesis Research 79, 189-200.

Xu, Y., Bruening, M.L., Watson, J.T. (2003 )Non-specific, on-probe cleanup methods for MALDI-MS samples. Mass Spectrom. Rev. 22, 429-440.

Chad R. Borges and J. Throck Watson. (2003) Recognition of Cysteine-Containing Peptides through Prompt Fragmentation of the 4-Dimethylaminophenylazophenyl-4'-maleimide Derivative During Analysis by MALDI-MS. Protein Science 12(7): 1567-72.

Borges, Chad R., Kuhn, Donald M., and Watson, J. Throck. (2003) Mass Mapping Sites of Nitration in Tyrosine Hydroxylase: Random versus Selective Nitration of Three Tyrosine Residues. Chemical Research in Toxicology. 16(4):536-540.

Qi,J., Hang, D., Rupp, M., Borges, C.R., Wu, W., Torng, E., and Watson. J.T. (2003) Automated Data Interpretation Based on the Concept of 'Negative Signature Mass' for Mass-Mapping Disulfide Structures of Cystinyl Proteins. (2003) J. Amer. Soc. Mass Spectrom. 14(9):1032-1038.

Xu, Y., Bruening, M., and Watson, J.T. (2004) Patterned monolayer/polymer films for analysis of dilute or salt-contaminated protein samples by MALDI-MS. Anal Chem. 75(2):185-190.

Borges, Chad R., Geddes, Timothy, Watson, J. Throck, and Kuhn, Donald M.(2003) Dopamine Biosynthesis Is Regulated by S-glutathionylation: Potential Mechanism of Tyrosine Hydroxylase Inhibition During Oxidative Stress. J Biol Chem. 277(50):48295-302.

Kuhn, D. M., Sadidi, M., Liu, X., Kreipke, C., Geddes, T., Borges, C., and Watson, J. T. (2002) Peroxynitrite-induced nitration of tyrosine hydroxylase: identification of tyrosines 423, 428, and 432 as sites of modification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and tyrosine-scanning mutagenesis. J Biol Chem. 277 (16) 14336-14342.

Qi, J., Wu, J., Somkuti, G., and Watson, JT (2001) Disulfide Structure of Sillucin, a Highly-Knotted, Cysteine-Rich Peptide by Cyanylation/Mass Mapping. Biochemistry 40(15): 4531-4538.

Sadagopan, N and Watson, JT. (2001) Mass Spectrometric Evidence for Fragmentation Mechanisms of Charged Derivatives of Peptides J. Amer. Soc. for Mass Spectrometry 12:399-409.

Watson, J. T. (2001) Trapping of intermediates during the refolding of recombinant human epidermal growth factor (hEGF) by cyanylation, and subsequent structural elucidation by mass spectrometry. Journal of Molecular Graphics and Modeling 19: 119-128.

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